#Multi measure plugin imagej how to#

Corresponds to the highest peak in the histogram. Modal Gray Value - Most frequently occurring gray value within the selection. Standard Deviation - Standard deviation of the gray values used to generate Gray=(red+green+blue)/3 if 'Unweighted RGB to Grayscale Conversion' is checked For RGB images, the mean is calulated by convertingĮach pixel to grayscale using the formula gray=0.299red+0.587green+0.114blue or the formula If Analyze>Calibrate was used to calibrate the image. Reported in calibrated units (e.g., optical density) The pixels in the selection divided by the number of pixels.

This is the sum of the gray values of all Mean Gray Value - Average gray value within the selection. Area is in calibrated units, such as square millimeters, if Analyze>Set Scale was used to spatially calibrate the image.

Use this dialog box to specify which measurements are recorded by Analyze>Measure andĪrea - Area of selection in square pixels.

#Multi measure plugin imagej how to#

Macro demonstrates how to use this feature.Ĭheck Add to Manager and the measured particles will be added to theįor each column in the results table, calculates and displays the mean, standard deviation, minimum and maximum of the values in that column.Įrases the results table and resets the measurement counter. The Record Starts option allows plugins and macros to recreate particle outlines When it is disabled, ImageJ finds the extent by flood filling. When this option is enabled, ImageJ finds the extent of each particle To measure particles enclosed by other particles. The best fit ellipse of each measured particles.Ĭheck Display results to have the measurements for each particle displayed in the 'Results' window.Ĭheck Clear Results to erase any previous measurement results.Ĭheck Summarize to display, in a separate window, the particle count, total particle area, average particle size,Ĭheck Exclude on Edges to ignore particles touching the edge of the image or selection.Ĭheck Include Holes to include interior holes.ĭisable this option to exclude interior holes and Select Masks to displayįilled outlines of the measured particles or Ellipses to display Select Outlines from the 'Show:' popup menu and ImageJ will open a window containing numbered A value of 1.0 indicates a perfect circle. The formula for circularity is 4pi(area/perimeter^2). Particles with circularity values outside the range specified in theĬircularity field are also ignored. Enter a single value in Size and particles smaller than that valueĪre ignored. Use the dialog box to configure the particle analyzer. Use Image>Adjust>Threshold to threshold an image. Resumes scanning until it reaches the end of the image or selection. The wand tool, measures it using the Measure command, fills it to make it invisible, then The image or selection until it finds the edge of an object. This command counts and measures objects in binary or thresholded images. The width of the columns in the 'Results' window can be adjusted by clicking on and dragging the vertical Measurements by right-clicking in the Results window and selecting The clipboard by selecting Edit>Copy All from the 'Results' window menu bar. To export the measurements as a tab-delimited text file, select File>Save As>Measurementsįrom the ImageJ menu bar or File>Save As from the 'Results' window menu bar. are calculated from the values of the pixels along the line. Standard deviation, mode, min, max and bounding rectangle (v1.34l or later). With line selections, the following parameters can be recorded: length, angle (straight lines only), mean, The weighting factors can be changed using Used to convert to from RGB to YUV, the color encoding The default weighting factors are the ones RGB pixels are converted toįormula V=(R+G+B)/3, or V=0.299R+0.587G+0.114B if 'Weighted RGB Conversions' isĬhecked in Edit>Option>Conversions. With RGB images, results are calculated using brightness values. Use the Analyze>Set Measurements command to specify what area statistics are recorded.

RecordsĬoordinates if one or more points have been defined using the point selection tool. Calculates line lengthĪnd angle if a line selection has been created using one of the three line selection tools. Has been selected using one of the first four tools in the tool bar. Home | contents | previous | next Analyze Menuīased on the selection type, calculates and displays either area statistics, line lengths and angles,Īrea statistics are calculated if there is no selection or if a subregion of the image